Enzymatic synthesis of an ezetimibe intermediate using carbonyl reductase coupled with glucose dehydrogenase in an aqueous-organic solvent system

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• 作者：Zhi-Qiang Liu^a ; Si-Chuan Dong^a ; Huan-Huan Yin^a ; Ya-Ping Xue^a ; Xiao-Ling Tang^a ; Xiao-Jian Zhang^a ; Jun-Yao He^b ; Yu-Guo Zheng^a ; ^{zhengyg@zjut.edu.cn}
• 关键词：Carbonyl reductase ; Glucose dehydrogenase ; Chiral intermediate of ezetimibe ; Dual-enzyme coupled system ; Aqueous-organic solvent system
• 刊名：Bioresource Technology
• 出版年：2017
• 出版时间：April 2017
• 年：2017
• 卷：229
• 期：Complete
• 页码：26-32
• 全文大小：783 K
• 卷排序：229

文摘

A newly synthesized SCR gene was expressed in E. coli for (S)-ET-5 production. The bioproduction of (S)-ET-5 in a biphasic solvent was first constructed. The (S)-ET-5 was achieved by bioconversion without extra addition of cofactor. The conversion of 99.1%, d.e. > 99.9% was achieved at 150 g L−1 ET-4.