Frequency analysis of HLA class I alleles in Iranian patients with progressive and non-progressive chronic lymphocytic leukemia

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• 作者：M. Hojjat-Farsangi ; S.M. Razavi ; R.A. Sharifian ; F. Shokri
• 刊名：Human Immunology
• 出版年：February, 2014
• 年：2014
• 卷：75
• 期：2
• 页码：170-175
• 全文大小：370 K

文摘

Chronic lymphocytic leukemia (CLL) is a malignant disorder of B cell origin, with low incidence in Asian populations. In this study we investigated the HLA-class I A and B allele frequencies in 87 Iranian CLL patients and 64 healthy controls using sequence specific primer-polymerase chain reaction (SSP-PCR) technique. Our results showed increased frequencies of HLA-A11:01 (p = 0.02) and HLA-B35:01 (p = 0.002) alleles and HLA-A11:01/B35:01 haplotype (p = 0.036) and decreased frequencies of HLA-A01:01 (p = 0.02), HLA-A26:01 (p = 0.03), HLA-B65:01 (p = 0.03) and HLA-B53:01 (p < 0.00001) alleles in CLL patients compared to the control group. Classification of the patients into non-progressive and progressive groups did not reveal significant differences for the frequency of any of the HLA-A and -B alleles or haplotypes between these two subtypes. Comparison between patients with immunoglobulin heavy chain variable region genes (IGHV) mutated (n = 56) and unmutated (n = 31) subtypes showed a significant increase in HLA-A32:01 (p = 0.05) and HLA-A33:01 (p = 0.05) alleles in IGHV unmutated patients compared to IGHV mutated patients. Similarly, a higher frequency of HLA-B52:01 (p = 0.037) alleles was observed in CD38⁺ compared with CD38^{鈭?/sup> patients. Our results obtained from an Iranian population indicate that CLL is associated with distinct HLA class I alleles and haplotypes some of which are linked to disease prognostic factors.}