Selective adsorption of acidic protein of bovine serum albumin onto sheet-like calcium hydroxyapatite particles produced by microreactor

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• 作者：Kazuhiko Kandori^a ; ^{kandori@cc.osaka-kyoiku.ac.jp" class="auth_mail} ; Hiroaki Hamazaki^a ; Mitsuhiro Matsuzawa^b ; Shigenori Togashi^b
• 关键词：Sheet-like calcium hydroxyapatite ; Selective adsorption of BSA molecules ; Microreactor ; Protein adsorption
• 刊名：Advanced Powder Technology
• 出版年：January, 2014
• 年：2014
• 卷：25
• 期：1
• 页码：354-359
• 全文大小：1345 K

文摘

The protein adsorption behavior onto sheet-like calcium hydroxyapatite Ca₁₀(PO₄)₆(OH)₂ (Hap) particles produced by microreactor was examined by using typical acidic bovine serum albumin (BSA) and basic lysozyme (LSZ). Since the sheet-like Hap particles were highly growth through b- (or a-) and c-axes, the particles had larger fraction of bc (or ac) crystal face. The saturated amounts of adsorbed BSA () for the sheet-like Hap nanoparticles were increased with increase in the particle size; i.e., showed a good linear relationship with the total surface area of bc and ac faces of each particle. This result strongly suggested that the sheet-like Hap particles progressed in b- and c-axes with large fraction of C sites on bc and ac particle faces and exhibit a high selective adsorption of BSA. On the contrary, very few LSZ molecules adsorbed onto sheet-like Hap particles. Hence the present study developed that the amounts of adsorbed protein, especially for BSA, can be controlled optimally by altering the size of sheet-like Hap particles. It was also found that the sheet-like Hap particles can be applied to separate completely BSA molecules from BSA-LSZ mixed solution.