The importance of MBs in behavioral rhythmicity remains controversial in Drosophila.
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The locomotor activity in MB-ablated flies is substantially rhythmic.
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Activation of MB neurons induces arrhythmic locomotor activity.
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We found that blocking of neurotransmission in MBs induces behavioral arrhythmicity.
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This study supports the notion that MB signaling is required for behavioral rhythms.
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