Overexpression of S-adenosylmethionine decarboxylase gene in rice increases polyamine level and enhances sodium chloride-stress tolerance

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• 作者：Roy ; Malabika ; Wu ; Ray
• 关键词：Polyamines ; Saline stress ; S-adenosylmethionine decarboxylase ; Transgenic rice
• 刊名：Plant Science
• 出版年：2002
• 出版时间：November, 2002
• 年：2002
• 卷：163
• 期：5
• 页码：987-992
• 全文大小：280 K

文摘

S-adenosylmethionine decarboxylase (SAMDC) is a key enzyme involved in the biosynthesis of the polyamines, viz. spermidine and spermine. SAMDC cDNA from Tritordeum was introduced into the rice (Oryza sativa L.) genome using an Agrobacterium-mediated transformation method. Transgenic rice plants showed normal growth and development. DNA blot analysis confirmed stable integration of the transgene. ABA-induced SAMDC gene expression was observed in RI transgenic plants under NaCl stress, and the transgenic plants showed increased seedling growth compared to non-transgenic controls. There was a three- to fourfold increase in spermidine and spermine levels in transformed plants under the NaCl stress.