High-yield soluble expression, purification and characterization of human steroidogenic acute regulatory protein (StAR) fused to a cleavable Maltose-Binding Protein (MBP)

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High-yield soluble expression, purification and characterization of human steroidogenic acute regulatory protein (StAR) fused to a cleavable Maltose-Binding Protein (MBP)

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作者：Nikolai N. Sluchanko^a ; ^{nikolai.sluchanko@mail.ru" class="auth_mail" title="E-mail the corresponding author} ; Kristina V. Tugaeva^a ; ^b ; Yaroslav V. Faletrov^c ; Dmitrii I. Levitsky^a ; ^d
关键词：Steroidogenic acute regulatory protein ; Soluble expression ; Maltose-Binding Protein ; Fluorescence ; Steroid hormones ; Cholesterol
刊名：Protein Expression and Purification
出版年：2016
出版时间：March 2016
年：2016
卷：119
期：Complete
页码：27-35
全文大小：1183 K

文摘

•: StAR protein has limited solubility and is mostly obtained through denaturation/refolding.
•: This is time-consuming, costly and may affect protein characteristics.
•: Fusing cleavable MBP to StAR confers its full solubility and facilitates purification.
•: The developed protocol results in native and aggregates-free StAR.
•: Capable of binding cholesterol, it is suitable for future investigations and biotechnology.

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