Heterogeneity of monoaminergic vesicular carriers: pharmacological evidence using MPP⁺ as a marker

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• 作者：Ruiu ; Stefania ; Piccardi ; Maria Paola ; Bocchetta ; Alberto ; Del Zompo ; Maria
• 关键词：MPP⁺ ; vesicular carriers ; monoamines ; pharmarological features ; central nervous system
• 刊名：Life Sciences
• 出版年：1996
• 出版时间：October 11, 1996
• 年：1996
• 卷：59
• 期：20
• 页码：1703-1710
• 全文大小：455 K

文摘

MPP⁺ production and uptake by dopaminergic terminals are critical steps in MPTP-induced Parkinson-like disorder. We reported evidence for a specific uptake of MPP⁺ by synaptic vesicles from mouse striatum. Its regional distribution suggests it as a marker of the dopamine vesicular carrier. We decided to further characterize such an MPP⁺ uptake. Tetrabenazine inhibits the dopamine uptake both in the striatum and in the cerebellum with similar Km values suggesting an identity of the vesicular carrier in these areas. On the contrary, ³H-MPP⁺ vesicular uptake had in the striatum a t of 60 sec, but was not detectable at any time in the cerebellum. Moreover, MPP⁺ inhibited the uptake of ³H-DA (Ki: 1.6 ± 0.03 μM) and ³H-NE (Ki: 2.6 ± 0.01 μM) in the striatum but not in the cerebellum, even at mmolar concentration. These pharmacological data indicate that in non-dopaminergic areas the monoamine carrier may be similar but not identical from that located in dopaminergic areas.