2¡ä-O-{[2,2-dimethyl-2-(2-nitrophenyl) acetyl] oxy} methyl protecting group for RNA synthesis

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• 作者：Ke Chen ; Wei Wang ; Dezhong Qu ; Haoting Zhao ; Wei Xiong ; Caijie Luo ; Menghui Yin ; Biliang Zhang
• 关键词：Chemical modification ; Solid phase synthesis of RNA ; 2&prime ; -OH protecting group ; RNA oligonucleotides ; SiRNA
• 刊名：Tetrahedron Letters
• 出版年：2013
• 出版时间：7 August, 2013
• 年：2013
• 卷：54
• 期：32
• 页码：4281-4284
• 全文大小：657 K

文摘

A new 2¡ä-OH protecting group, {[2,2-dimethyl-2-(2-nitrophenyl)acetyl]oxy} methyl (DAM), has been successfully applied to RNA oligonucleotides synthesis with high coupling efficiency. The 2¡ä-O-DAM method is fully compatible with reagents and conditions employed in automated solid phase synthesis of RNA. Moreover, it can be easily removed via reduction in near-neutral solution.