Cannabidiol (CBD) induces functional Tregs in response to low-level T cell activation

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• 作者：Saphala Dhital^a ; John V. Stokes^b ; Nogi Park^b ; Keun Seok Seo^b ; Barbara L.F. Kaplan^a ; ^{barbara.kaplan@msstate.edu}
• 关键词：BCS ; bovine calf serum ; BFA ; Brefeldin A ; CBD ; cannabidiol ; CB1 ; cannabinoid receptor 1 ; CB2 ; cannabinoid receptor 2 ; cpm ; counts per minute ; DW ; deionized water ; ELISA ; enzyme linked immunosorbent assay ; FOXP3 ; forkhead box P3 ; FVD ; fixable viability dye ; IFN ; interferon ; IL ; interleukin ; iTregs ; inducible T regulatory T cells ; MMC ; mitomycin C ; MOG ; myelin oligodendrocyte glycoprotein ; PMA ; phorbol 12-myristate 13-acetate ; RT-PCR ; reverse transcription-polymerase chain reaction ; s3/28 ; soluble
• 刊名：Cellular Immunology
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：312
• 期：Complete
• 页码：25-34
• 全文大小：1812 K
• 卷排序：312

文摘

CBD induces CD25 and FOXP3 on CD4+ T cells in response to Us/o, S/o and s3/28 stimulation. CBD is most effective in inducing CD4+CD25+FOXP3+ T cells in response to Us/o. Us/o + CBD increased FOXP3 expression on CD4+ cells derived from FOXP3-GFP mice. Us/o + CBD induced FOXP3 in pre-purified CD4+CD25− and CD4+CD25+ T cells. Us/o + CBD-induced CD4+CD25+ T cells suppressed responder cell proliferation.