Preparation of cell-culturing glass surfaces that release branched polyethyleneimine triggered by thiol-disulfide exchange

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• 作者：Aya Mizutani Akimoto ; ^{akimoto@cross.t.u-tokyo.ac.jp} ; Tohru Takarada ; Mizuo Maeda
• 关键词：Polyethyleneimine ; Cysteine ; Thiol&ndash ; disulfide exchange ; Disulfide linkage ; Cell-culturing substrates
• 刊名：Colloids and Surfaces B: Biointerfaces
• 出版年：2013
• 出版时间：1 March, 2013
• 年：2013
• 卷：103
• 期：Complete
• 页码：360-365
• 全文大小：953 K

文摘

To develop a chemical stimulus-responsive substrate for culturing cells, polyethyleneimine (PEI) having a pyridyl disulfide moiety was attached via disulfide linkages to a glass coverslip modified with a silane coupling agent having a thiol group. The surface modification was confirmed by X-ray photoelectron spectroscopy and zeta potential analysis. The obtained surface exhibited sufficiently high cell adhesiveness. Zeta potential measurements revealed that the PEI derivatives were released from the surface through thiol-disulfide exchange when the modified glass coverslip was immersed in a neutral pH buffer containing cysteine. The cell viability assay demonstrated that this chemical stimulus was substantially nontoxic to 293T cells. Because PEI is a widely used transfection reagent, this functional glass coverslip would be potentially useful as an experimental platform for reverse transfection.