文摘
To evaluate the effect of supplementing single-step embryo culture medium with insulin on human embryo development.DesignComparative study.SettingTwo private centers.Patient(s)The study involved a sibling oocyte split of 5,142 retrieved oocytes from 360 patients.Intervention(s)Sibling oocytes split after intracytoplasmic sperm injection for culture from day 0 through day 5 or 6 in insulin-supplemented or control medium. Women were split to receive their embryos from insulin-supplemented or control medium.Main Outcome Measure(s)Clinical pregnancy rate.Result(s)There were significantly higher rates of clinical, ongoing, and twin pregnancies in the insulin-supplemented arm than in the control arm. On day 3, embryo quality and compaction were higher in insulin-supplemented medium. On day 5, insulin supplementation showed higher rates of blastocyst formation, quality, and cryopreservation.Conclusion(s)Insulin supplementation of single-step embryo culture medium from day 0 through day 5 or 6 improved clinical pregnancy rate and human embryo development. However, these findings need further confirmation through a multicenter randomized controlled trial that may include other patient populations and different culture media.
