文摘
In this study, the anticoagulant, hemorrhagic and antithrombotic effects of glycosaminoglycans from Apostichopus japonicus (AHG) and its depolymerized products (DAHGs) were firstly investigated and compared with that of low molecular weight heparin. AHG and DAHGs exhibited significant prolongation of APTT in vitro. In vivo antithrombus assays of AHG and DAHGs on mice further concluded that AHG and DAHGs could prevent the venous thrombus formation and platelet aggregation effectively. Moreover, AHG and DAHGs could markedly prevent the ex vivo and in vivo thrombus formation at their effective dosage on rats. They functioned in molecular weight and dosage dependent manners. The activities would decrease with the decreasing molecular weight. However, the anticoagulant dose/hemorrhagic dose ratio of DAHGs were higher than that of AHG and LMWH, suggesting that depolymerized glycosaminoglycans could show the antithrombotic effects with less bleeding risk. Compared with other DAHGs, DAHG-2 showed good antithrombotic and anticoagulant properties and caused less bleeding, making it a promising candidate for the replacement of heparin and LMWH.
