Interaction between non-anionic phospholipids and cytochrome c induced by reactive oxygen species

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• 作者：Nazha Sidahmed-Adrar ; Catherine Marchetti ; Dominique Bonnefont-Rousselot ; Juliette Thariat ; Delphine Onidas ; Daniel Jore ; Monique Gardes-Albert ; Fabrice Collin
• 关键词：Cytochrome c ; Phospholipid ; Reactive oxygen species ; Peroxidation ; Mass spectrometry
• 刊名：Chemistry and Physics of Lipids
• 出版年：2010
• 出版时间：June 2010
• 年：2010
• 卷：163
• 期：6
• 页码：538-544
• 全文大小：621 K

文摘

The oxidative interaction of cytochrome c (Cyt c) with liposomes of Palmitoyl Linoleyl Phosphatidyl Choline (PLPC) initiated by radio-induced free radicals was investigated. Results showed that the peroxidation of PLPC is decreased in the presence of Cyt c, meaning that this latter is the preferential target of hydroxyl radicals. In addition, when Cyt c was incubated with peroxidized PLPC, it was found to be able to decompose hydroperoxides of PLPC into hydroxides. The peroxidase activity of Cyt c proceeded via the opening of the tertiary structure of Cyt c, as suggested by the loss of the sixth coordination bond of the heme-iron. Even if it is known to preferentially interact with cardiolipin, this work shows that Cyt c is also able to interact with hydroperoxide species of non-anionic phospholipids.