Sensitive electrochemical microbial biosensor for p-nitrophenylorganophosphates based on electrode modified with cell surface-displayed organophosphorus hydrolase and ordered mesopore carbons

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Sensitive electrochemical microbial biosensor for p-nitrophenylorganophosphates based on electrode modified with cell surface-displayed organophosphorus hydrolase and ordered mesopore carbons

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作者：Xiangjiang Tang^a ; ¹ ; Tingting Zhang^a ; ^b ; ¹ ; Bo Liang^a ; Dongfei Han^a ; Lingxing Zeng^c ; Cheng Zheng^c ; Tie Li^b ; Mingdeng Wei^c ; Aihua Liu^a ; ^{liuah@qibebt.ac.cn" class="auth_mail}
关键词：Bacterial surface display ; Organophosphorus hydrolase ; p-nitrophenyl organophosphates ; Ordered mesoporous carbons ; Electrochemical microbial biosensor ; Amperometric detection
刊名：Biosensors and Bioelectronics
出版年：15 October 2014
年：2014
卷：60
期：Complete
页码：137-142
全文大小：800 K

文摘

•: OPH displayed on the surface of Escherichia coli with excellent enzyme activity and stability.
•: Sensitive electrochemical microbial biosensor for p-nitrophenyl OPs was developed with ordered mesoporous carbons and OPH-bacteria.
•: The amperometric biosensor was capable of detecting trace p-nitrophenyl OPs with high specificity.