Molecular staging of marine medaka: A model organism for marine ecotoxicity study

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• 作者：Xueping Chen^a ; Li Li^a ; Jinping Cheng^a ; Leo Lai Chan^a ; Da-Zhi Wang^b ; Ke-Jian Wang^b ; Michael E. Baker^c ; Gary Hardiman^c ; Daniel Schlenk^d ; Shuk Han Cheng^a ; ^{bhcheng@cityu.edu.hk"" rel=""nofollow}
• 关键词：Medaka ; Oryzias melastigma ; Molecular staging ; In situ hybridization ; Immunostaining
• 刊名：Marine Pollution Bulletin
• 出版年：2011
• 出版时间：2011
• 年：2011
• 卷：63
• 期：5-12
• 页码：309-317
• 全文大小：2014 K

文摘

Oryzias melastigma, also called O. dancena, is becoming a very useful model for estuarine and marine ecotoxicity studies. With O. melastigma being adopted by ILSI Health and Environmental Science Institute (HESI) for embryo toxicity testing, improved knowledge of biomarker based embryonic development becomes especially important for mechanism-based toxicity evaluations. Using whole mount in situ hybridization and immunostaining techniques together with widely used molecular markers, this study describes the molecular development of marine medaka embryos, focusing on the brain, eye, heart, pectoral fin, pancreas, liver, muscle and neuron system. These organs are targets of environmental pollutants that disrupt normal embryonic development in medaka and other fish.