A new isoluminol reagent for chemiluminescence labeling of proteins

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• 作者：Aless ; ro Palmioli ; Marco Crisma ; Cristina Peggion ; PierNatale Brusasca ; Davide Zanin ; Andrea Dal Corso ; Paolo Ingallinella ; Francesco Peri
• 关键词：Isoluminol ; Activated ester ; Lactone ; Protein conjugation ; Chemiluminescence ; Labeled antibodies
• 刊名：Tetrahedron Letters
• 出版年：2013
• 出版时间：14 August, 2013
• 年：2013
• 卷：54
• 期：33
• 页码：4446-4450
• 全文大小：841 K

文摘

We report the synthesis and the characterization of N-(4-aminobutyl)-N-ethyl-isoluminol (ABEI) macrocyclic lactone, an activated ester with an unusual macrocyclic structure, and its use for efficient ABEI conjugation to proteins. Compared to the equivalent reagent normally used for chemiluminescence protein labeling, the ABEI macrocyclic lactone displays improved chemical properties, including stability and reactivity. We show the simple synthesis and the use of ABEI macrocyclic lactone for efficient chemiluminescence labeling of monoclonal antibody mixtures currently used in clinical immunodiagnostic assays for the detection of the HIV p24 antigen in patients.