Ire1 regulated XBP1 mRNA splicing is essential for the unfolded protein response (UPR) in Drosophila melanogaster

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• 作者：Nongluk Plongthongkum ; Niwed Kullawong ; Sakol Panyim ; Witoon Tirasophon
• 关键词：Unfolded protein response (UPR) ; Endoplasmic reticulum (ER) ; Inositol required 1 (Ire1) ; X-box binding protein (XBP1) ; Drosophila melanogaster
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2007
• 出版时间：16 March 2007
• 年：2007
• 卷：354
• 期：3
• 页码：789-794
• 全文大小：285 K

文摘

The ability of cells to survive and recover from deteriorating effects of endoplasmic reticulum (ER) stress relies on the unfolded protein response (UPR). The signaling pathway of Ire1p mediate mRNA splicing plays diverge role in UPR response in different organisms from yeast to mammals. Here, we report that Ire1p mediated XBP1 mRNA splicing mechanism is extremely conserved and exerts a critical role for modulating Xbp1 protein synthesis in Drosophila melanogaster. This system is operative in Drosophila S2 cells as a prominent mechanism to mediate transcriptional activation of UPR responsive genes during ER stress.