Synergism of regulatory elements in 蟽^B- and 蟽^A-dependent promoters enhances recombinant protein expression in Bacillus subtilis

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• 作者：Teva Phanaksri¹ ; Plearnpis Luxananil² ; Sakol Panyim¹ ; ³ ; Witoon Tirasophon¹ ; ^{witoon.tir@mahidol.ac.th" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Bacillus subtilis ; Double promoter ; Synergism ; 蟽A-dependent promoter ; 蟽B-dependent promoter ; Recombinant protein expression ; Green fluorescent protein
• 刊名：Journal of Bioscience and Bioengineering
• 出版年：2015
• 出版时间：October 2015
• 年：2015
• 卷：120
• 期：4
• 页码：470-475
• 全文大小：1229 K

文摘

Strong promoter is an essential factor for production of recombinant protein in various expression systems including Bacillus subtilis. In this study, we described a strategy to improve the expression efficiency using synthetic double promoter. Assembly of the conserved elements from 蟽^B- and 蟽^A-dependent promoters constitutively improved the yield of recombinant protein approximately 2–3-fold in both exponential and stationary growth phase. The synergistic effect in the double promoter was observed only when 蟽^B-promoter was located upstream to 蟽^A-promoter but independent to its orientation. A conserved element in either −10 or −35 box of 蟽^B-promoter is sufficient to promote the synergism. Hence, this simple strategy of promoter engineering could be an effective way to generate a pool of strong constitutive promoters applicable for heterologous protein expression in B. subtilis in the future.