Enhancing the production of recombinant acidic α-amylase and phytase in Pichia pastoris under dual promoters [constitutive (GAP) and inducible (AOX)] in mixed fed batch high cell density cultivation

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Enhancing the production of recombinant acidic α-amylase and phytase in Pichia pastoris under dual promoters [constitutive (GAP) and inducible (AOX)] in mixed fed batch high cell density cultivation

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作者：Deepak Parashar ; T. Satyanarayana ; ^{tsnarayana@gmail.com" class="auth_mail" title="E-mail the corresponding author}
关键词：Pichia pastoris ; Methanol ; Glycerol ; High cell density ; Fed batch fermentation ; Specific productivity
刊名：Process Biochemistry
出版年：2016
出版时间：October 2016
年：2016
卷：51
期：10
页码：1315-1322
全文大小：2081 K

文摘

•: Strategy has been developed to express proteins under dual promoters (AOX and GAP).
•: Dual promoters led to improvement in productivity of α-amylase and phytase.
•: The presence of multiple copies of Amy and Phy did not affect growth of P. pastoris.

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