Thiolated polyethylene oxide as a non-fouling element for nano-patterned bio-devices

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• 作者：Patrí ; cia Lisboa ; Andrea Valsesia ; Pascal Colpo ; Douglas Gillil ; Giacomo Ceccone ; Andri Papadopoulou-Bouraoui ; Hubert Rauscher ; Fabiano Reniero ; Claude Guillou ; Franç ; ois Rossi
• 关键词：Polyethylene oxide ; Thiol ; Nanotopography ; Protein adsorption
• 刊名：Applied Surface Science
• 出版年：2007
• 出版时间：15 March 2007
• 年：2007
• 卷：253
• 期：10
• 页码：4796-4804
• 全文大小：718 K

文摘

This work describes the synthesis of a thiolated polyethylene oxide that self-assembles on gold to create a non-fouling surface. Thiolated polyethylene oxide was synthesised by reacting 16-mercaptohexadecanoic acid with polyethylene glycol mono methyl ether. The coverage of the thiolated polyethylene oxide on gold was studied by cyclic voltammetry, and the modified surfaces were characterised by X-ray photoelectron spectroscopy and ellipsometry. Protein resistance was assessed using quartz crystal microbalance. Results showed a non-fouling character produced by the thiolated polyethylene oxide. The synthesised product was used as the passivation layer on nano-patterned surfaces consisting of arrayed nano-spots, fabricated by plasma based colloidal lithography. The specific adsorption of anti-bovine serum albumin in the mercaptohexadecanoic acid spots was verified by atomic force microscopy.