Penicillin acylase and O-aryloxycarbonyl hydroxamates: Two acyl-enzymes, one leading to hydrolysis, the other to inactivation

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• 作者：S.A. Adediran ; R.F. Pratt ; ^{rpratt@wesleyan.edu}
• 关键词：Penicillin acylase ; N-terminal hydrolase ; O-Aryloxycarbonyl hydroxamates ; Enzyme kinetics ; Enzyme inactivation ; Enzyme mechanism
• 刊名：Archives of Biochemistry and Biophysics
• 出版年：2017
• 出版时间：15 January 2017
• 年：2017
• 卷：614
• 期：Complete
• 页码：65-71
• 全文大小：612 K
• 卷排序：614

文摘

Potent covalent inhibitors of penicillin acylase. Two orientations of inhibitors binding to the active site. Two distinct acyl-enzyme intermediates, one leading to hydrolysis, the other to enzyme inactivation. Asymmetric carbonates as very versatile enzyme inhibitors.