Overexpression of the TIR-X gene results in a dwarf phenotype and activation of defense-related gene expression in Arabidopsis thaliana

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• 作者：Hiroaki Kato^a ; Tamao Saito^b ; Hidetaka Ito^c ; ^d ; Yoshibumi Komeda^e ; Atsushi Kato^c ; ^{atsushi@sci.hokudai.ac.jp" class="auth_mail}
• 关键词：ADR1 ; activated disease resistance 1 ; avr ; avirulence gene ; BGL2 ; beta-1 ; 2-glucanase 2 ; BON1-1 ; bonzai1-1 ; CSB3 ; constitutive subtilisin 3 ; CC ; coiled-coil ; CPR ; constitutive expresser of pathogenesis-related gene ; DND ; defense no death ; EDS1 ; enhanced disease susceptibility 1 ; EF1a ; elongation factor 1a ; ET ; ethylene ; GUS尾 ; -glucuronidase ; JA ; jasmonic acid ; LRR ; leucine-rich repeat ; NBS ; nucleotide-binding site ; NPR1 ; non-expresser of PR genes 1 ; PAD4 ; phytoalexin deficient 4 ; PDF1.2A ; plant
• 刊名：Journal of Plant Physiology
• 出版年：15 March, 2014
• 年：2014
• 卷：171
• 期：6
• 页码：382-388
• 全文大小：2317 K

文摘

The Arabidopsis genome encodes various proteins with a Toll/interleukin-1 receptor (TIR) domain. Many of these proteins also contain nucleotide-binding site (NBS) and leucine-rich repeat (LRR) domains and function as resistance (R) proteins. However, the protein encoded by At2g32140 (a TIR-X gene) contains a TIR domain but lacks NBS and LRR domains. We found that transgenic plants overexpressing At2g32140 displayed a dwarf phenotype and showed increased expression of defense-related genes. In general, the growth defect caused by activation of defense responses is suppressed under high-temperature conditions. However, transgenic plants overexpressing At2g32140 displayed a much stronger dwarf phenotype at 28 掳C than at 22 掳C. This dwarf phenotype was suppressed under the combination with known salicylic-acid pathway mutants. These findings suggest that At2g32140 encodes a protein involved in the plant defense response.