Improved determination of urinary cortisol and cortisone, or corticosterone and 11-dehydrocorticosterone by high-performance liquid chromatography with ultraviolet absorbance detection

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• 作者：Magali Hay ; Pierre Morm&#xe8 ; de
• 关键词：Cortisol ; Cortisone ; Corticosterone ; 11-Dehydrocorticosterone
• 刊名：Journal of Chromatography B: Biomedical Sciences and Applications
• 出版年：1997
• 出版时间：21 November 1997
• 年：1997
• 卷：702
• 期：1-2
• 页码：33-39
• 全文大小：136 K

文摘

A sensitive assay was developed for the determination of low levels of free (unconjugated) glucocorticoids in human, swine (cortisol and cortisone) and rat urine (corticosterone and 11-dehydrocorticosterone), using solid-phase extraction and HPLC with UV absorbance detection (254 nm). Precise quantitation is allowed by the use of internal standards (dexamethasone for swine urine and Reichstein's substance S for rat urine). This simple method allows the use of small urine samples (less than 2 ml), and is suitable for a wide range of applications in human and animal clinical and physiological studies.