A novel thiol-affinity micropipette tip method using zinc(II)-cyclen-attached agarose beads for enrichment of cysteine-containing molecules

网站地图 | English | 公务邮箱

About the library

Background
History
Leadership
Organization

Opening Hours
Collections
Help Via Email

Electronic Information Resources

A novel thiol-affinity micropipette tip method using zinc(II)-cyclen-attached agarose beads for enrichment of cysteine-containing molecules

详细信息查看全文

作者：Hiroshi Kusamoto^a ; Akio Shiba^a ; Norinao Koretake^a ; Haruto Fujioka^b ; Yuhzo Hieda^b ; Emiko Kinoshita-Kikuta^a ; Eiji Kinoshita^a ; ^{kinoeiji@hiroshima-u.ac.jp" class="auth_mail" title="E-mail the corresponding author} ; Tohru Koike^a ; ^{tkoike@hiroshima-u.ac.jp" class="auth_mail" title="E-mail the corresponding author}
关键词：HPLC ; high-performance liquid chromatography ; HEPES ; 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ; Bis-tris ; bis(2-hydroxyethyl)imino-tris(hydroxymethyl)methane ; TCEP ; tris(2-carboxyethyl)phosphine ; AcCysNH2 ; N-acetylcysteine amide
刊名：Journal of Chromatography B, Analytical Technologies in the Biomedical and Life Sciences
出版年：2016
出版时间：15 September 2016
年：2016
卷：1031
期：Complete
页码：195-201
全文大小：1371 K

文摘

•: A novel thiol-affinity micropipette tip method was developed.
•: Thiol compounds captured by the thiol-affinity micropipette tip are stable under air.
•: The separation can be conducted by using aqueous solutions at room temperature.
•: The operation time from sample addition to elution is less than 15 min.
•: The thiol-affinity agarose beads are stable for multiple use and long-term storage.

NGLC 2004-2010.National Geological Library of China All Rights Reserved.
Add:29 Xueyuan Rd,Haidian District,Beijing,PRC. Mail Add: 8324 mailbox 100083
For exchange or info please contact us via email.