Palmitate induces RIP1/RIP3-dependent necrosis via MLKL-mediated pore formation in the plasma membrane of RAW 264.7 cells

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• 作者：Seong Keun Kim¹ ; Mihee Yun¹ ; Gimoon Seo ; Ji-Young Lee ; Seong-Beom Lee ; ^{sblee@catholic.ac.kr}
• 关键词：Palmitate ; RIP1 ; RIP3 ; MLKL ; Necrosis ; RAW 264.7 cells
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2017
• 出版时间：8 January 2017
• 年：2017
• 卷：482
• 期：2
• 页码：359-365
• 全文大小：1240 K
• 卷排序：482

文摘

The down-regulation of RIP1 or RIP3 by siRNA transfection protected the cells from palmitate-induced cell death in RAW 264.7 cells. MLKL was phosphorylated at the serine residue and translocated to the plasma membrane in palmitate-treated RAW 264.7 cells. The down-regulation of MLKL by siRNA transfection significantly inhibited palmitate-induced pore formation in the plasma membrane, resulting in protection against palmitate-induced necrotic cell death in RAW 264.7 cells.