Hydroxyurea-induced replication stress causes poly(ADP-ribose) polymerase-2 accumulation and changes its intranuclear location in root meristems of Vicia faba

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• 作者：Dorota Rybaczek ^{dorota.rybaczek@gmail.com" class="auth_mail" title="E-mail the corresponding author doryb@biol.uni.lodz.pl" class="auth_mail" title="E-mail the corresponding author}
• 关键词：DDR ; DNA Damage Response ; dNTP ; deoxyribonucleoside triphosphates ; DSBs ; double-Strand Breaks ; TEM ; transmission electron microscopy ; HU ; hydroxyurea ; NAD+ ; nicotinamide adenine dinucleotide ; NE ; nuclear envelope ; NER ; nucleotide excision repair ; pADPRT ; poly(ADP-ribosyl) transferase ; PAR ; poly(ADP-ribose) ; PARG ; poly(ADP-ribose) glycohydrolase ; PARPs ; poly(ADP-ribose) polymerases ; PARP-2 ; poly(APD-ribose) polymerase-2 ; PCC ; premature chromosome condensation ; POLR2A ; polymerase (RNA) II (DNA dir
• 刊名：Journal of Plant Physiology
• 出版年：2016
• 出版时间：1 July 2016
• 年：2016
• 卷：198
• 期：Complete
• 页码：89-102
• 全文大小：3121 K

文摘

Replication stress induced by 24 and 48 h exposure to 2.5 mM hydroxyurea (HU) increased the activity of poly(ADP-ribose) polymerase-2 (PARP-2; EC 2.4.2.30) in root meristem cells of Vicia faba. An increase in the number of PARP-2 foci was accompanied by their delocalization from peripheral areas to the interior of the nucleus. Our results indicate that the increase in PARP-2 was connected with an increase in S139-phosphorylated H2AX histones. The findings suggest the possible role of PARP-2 in replication stress. We also confirm that the intranuclear location of PARP-2 depends on the duration of HU-induced replication stress, confirming the role of PARP-2 as an indicator of stress intensity. Finally, we conclude that the more intense the HU-mediated replication stress, the greater the probability of PARP-2 activation or H2AXS139 phosphorylation, but also the greater the chance of increasing the efficiency of repair processes and a return to normal cell cycle progression.