Extracellular expression of alkali tolerant xylanase from Bacillus subtilis Lucky9 in E. coli and application for xylooligosaccharides production from agro-industrial waste

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• 作者：Siyuan Chang^a ; Yalan Guo^a ; Bin Wu^a ; ^b ; ^{wubin1977@njtech.edu.cn} ; Bingfang He^b ; ^c
• 关键词：Xylanase ; Extracellular expression ; Arabinose ; Alkali stable ; Corncob ; Xylooligosaccharides
• 刊名：International Journal of Biological Macromolecules
• 出版年：2017
• 出版时间：March 2017
• 年：2017
• 卷：96
• 期：Complete
• 页码：249-256
• 全文大小：1564 K
• 卷排序：96

文摘

Fusing pelB signal peptide was suitable for secretion xylanase to the culture medium. Additional arabinose was used as extra carbon source and inducer for enhancing extracellular production of xylanase. The xylanase was high pH stability (80%) over a broad pH range of 6.0–9.0 for 24 h. A high XOS production (8.7 mg/mL) of corncob was observed with extracellular xylanase.