Activity control of autodisplayed proteins on the same outer membrane layer of E. coli by using Z-domain/streptavidin/and lipase/foldase systems
文摘
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Outer membrane (OM) fractions were isolated after autodisplay of model proteins on OM of E. coli.

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OM Layer were made by mixing OM fractions of two kinds of autodisplayed proteins with controlled ratio.

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Two model protein-pairs were selected to be Z-domain-streptavidin and lipase-foldase.

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Each protein on OM layer were determined to have relative activity according to the mixing ratio.

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Activity of lipase could be optimized according to the mixing ratio of foldase in OM layer.

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