Hypouricemic effect of allopurinol are improved by Pallidifloside D based on the uric acid metabolism enzymes PRPS, HGPRT and PRPPAT

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• 作者：Hong-Gang Li^a ; ¹ ; Pi-Yong Hou^a ; ¹ ; Xi Zhang^a ; ¹ ; Yi He^a ; Jun Zhang^a ; Shu-Qing Wang^a ; Samantha Anderson^b ; Yan-Wen Zhang^a ; Xiao-Hui Wu^a ; ^b ; ^{longhui804@163.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：PRPS ; phosphoribosyl pyrophosphate synthetase ; HGPRT ; hypoxanthine-guanine phosphoribosyl transferase ; PRPPAT ; phosphoribosyl pyrophosphate amino-transferase ; PRPP ; 5-ribose phosphate-1-pyrophosphoric acid ; XOD ; xanthine oxidase ; IMP ; inosine monophosphate ; AMP ; adenine monophosphate ; GMP ; guanine monophosphate ; EtOH ; ethyl alcohol ; BuOH ; n-butyl alcohol ; HR-ESI-MS ; high-resolution mass spectrometer ; NMR ; nuclear magnetic resonance ; UA ; uric acid ; NC ; normal control
• 刊名：Fitoterapia
• 出版年：2016
• 出版时间：September 2016
• 年：2016
• 卷：113
• 期：Complete
• 页码：1-5
• 全文大小：450 K

文摘

Allopurinol is a commonly used medication to treat hyperuricemia and its complications. Pallidifloside D, a saponin glycoside constituent from the total saponins of Smilax riparia, had been proved to enhanced hypouricemic effect of allopurinol based on uric acid metabolism enzyme XOD. In this study, we evaluated whether Pallidifloside D (5 mg/kg) enhanced hypouricemic effect of allopurinol (5 mg/kg) related to others uric acid metabolism enzymes such as PRPS, HGPRT and PRPPAT. We found that, compared with allopurinol alone, the combination of allopurinol and Pallidifloside D significantly up-regulated HGPRT mRNA expression and down-regulated the mRNA expression of PRPS and PRPPAT in PC12 cells (all P < 0.01). These results strongly suggest that hypouricemic effect of allopurinol are improved by Pallidifloside D via numerous mechanisms and our data may have a potential value in clinical practice in the treatment of gout and other hyperuricemic conditions.