Robust l-malate bienzymatic biosensor to enable the on-site monitoring of malolactic fermentation of red wines

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• 作者：Pablo Gimé ; nez-Gó ; mez^a ; Manuel Gutié ; rrez-Capitá ; n^a ; ^{manuel.gutierrez@csic.es} ; Fina Capdevila^b ; Anna Puig-Pujol^b ; Cesar Ferná ; ndez-Sá ; nchez^a ; Cecilia Jimé ; nez-Jorquera^a
• 关键词：Amperometric bienzymatic biosensor ; Electrosynthesized polypyrrole membrane ; Immobilization of redox mediator ; l-malic acid analysis ; Malolactic fermentation
• 刊名：Analytica Chimica Acta
• 出版年：2017
• 出版时间：15 February 2017
• 年：2017
• 卷：954
• 期：Complete
• 页码：105-113
• 全文大小：1641 K
• 卷排序：954

文摘

Bienzymatic electrochemical biosensor for l-malate. Malate dehydrogenase and diaphorase enzymes entrapped into an electrosynthesized polypyrrole film. Optimized redox mediator also incorporated into the polymeric film. Long-term stability for over 37 days. Application to l-malic acid determination during the malolactic fermentation of red wines.