Correlating the structures and activities of the resting oxidized and native intermediate states of a small laccase by paramagnetic NMR

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作者：Michael C. Machczynski ; ^{machczym@newpaltz.edu" class="auth_mail" title="E-mail the corresponding author} ; Jeffrey T. Babicz Jr.¹
关键词：Paramagnetic NMR ; Oxidase ; Copper ; Native intermediate ; Exchange coupling ; Laccase
刊名：Journal of Inorganic Biochemistry
出版年：2016
出版时间：June 2016
年：2016
卷：159
期：Complete
页码：62-69
全文大小：869 K

文摘

•: The native intermediate state of the small laccase persists for months at 4 °C and pH 7.
•: An isolated Type-3 copper site produces the ¹H NMR spectrum of the resting oxidized state.
•: The site of three coupled Cu ions produces the ¹H NMR spectrum of the native intermediate.
•: A method for calculating the chemical shifts in an oxidized Cu₃ cluster is developed.
•: The energy of the quartet state supports the model of a hydroxo ligand at the Type-2 site.