Combined use of peptide ion and normalized delta scores to evaluate milk authenticity by ion-trap based proteomics coupled with error tolerant searching

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• 作者：Donatella Nardiello ; ^{donatella.nardiello@unifg.it} ; Anna Natale ; Carmen Palermo ; Maurizio Quinto ; Diego Centonze
• 关键词：Normalized delta score ; Peptide ion score ; Ion-trap ; Enzymatic digestion ; Collision-Induced Dissociation (CID) ; Milk authenticity
• 刊名：Talanta
• 出版年：2017
• 出版时间：1 March 2017
• 年：2017
• 卷：164
• 期：Complete
• 页码：684-692
• 全文大小：516 K
• 卷排序：164

文摘

Database post-processing validation of MS/MS data sets in ion-trap proteomics. Combined peptide ion and normalized delta scores for confident sequence assignments. Error tolerant searching to maximize sequence coverage. Milk authenticity by identification of species-specific peptides. Direct analysis of tryptic/chymotryptic milk digests, without 2D-PAGE.