Hyperspectral Raman imaging of human prostatic cells: An attempt to differentiate normal and malignant cell lines by univariate and multivariate data analysis

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• 作者：P. Musto^a ; ^{pellegrino.musto@ipcb.cnr.it} ; A. Calarco^b ; M. Pannico^a ; P. La Manna^a ; S. Margarucci^b ; A. Tafuri^c ; G. Peluso^b
• 关键词：Prostate cancer ; Single cell spectroscopy ; Raman spectroscopy ; Raman imaging ; Chemometrics
• 刊名：Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy
• 出版年：2017
• 出版时间：15 February 2017
• 年：2017
• 卷：173
• 期：Complete
• 页码：476-488
• 全文大小：1919 K
• 卷排序：173

文摘

Hyperspectral Raman images of normal and malignant prostatic cells were studied. The analysis was focused on the ν(CH) region (2800–3100 cm− 1). Univariate and multivariate chemometric methods were applied. The tumor cells exhibited a significant increase of the cytoplasmatic lipid fraction. Significant and reproducible differences between normal and tumor cells were identified.