Fork restart protein, PriA, binds around oriC after depletion of nucleotide precursors: Replication fork arrest near the replication origin

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作者：Taku Tanaka^a ; Yasumasa Nishito^b ; Hisao Masai^a ; ^{masai-hs@igakuken.or.jp" class="auth_mail" title="E-mail the corresponding author}
关键词：Genome stability ; Stalled replication fork ; oriC ; PriA ; eCOMB
刊名：Biochemical and Biophysical Research Communications
出版年：2016
出版时间：12 February 2016
年：2016
卷：470
期：3
页码：546-551
全文大小：1878 K

文摘

•: Upon thymine starvation, PriA protein binds around oriC, suggesting replication forks stall preferentially near oriC shortly after initiation.
•: PriA dissociates from oriC upon readdition of thymine to thymine-starved cells.
•: BrdU incorporation assays show fork restart at around oriC after release, consistent with the fork stall near oriC.
•: Specific replication fork pause/arrest sites were detected at ∼50 kb away from oriC in unperturbed growth.