Efficient production of Arthromyces ramosus peroxidase by Aspergillus awamori

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• 作者：Lokman ; B. Christien ; Joosten ; Vivi ; Hovenkamp ; Jacqueline ; Gouka ; Robin J. ; Verrips ; C. Theo ; et. al.
• 关键词：Aspergillus awamori ; Peroxidase ; Heterologous protein production ; Heme limitation
• 刊名：Journal of Biotechnology
• 出版年：2003
• 出版时间：June 26, 2003
• 年：2003
• 卷：103
• 期：2
• 页码：183-190
• 全文大小：328 K

文摘

The heterologous production of Arthromyces ramosus peroxidase (ARP) was analysed in the filamentous fungus Aspergillus awamori under control of the inducible endoxylanase promoter. Secretion of active ARP was achieved up to 800 mg l⁻¹ in shake flask cultures. Western blot analysis showed that an rARP product of the correct molecular weight was produced. In contrast to several other studies about heterologous production of heme containing peroxidases, our results suggest that in A. awamori no heme limitation exists during overproduction of ARP.