The expression and purification of WSSV134 from white spot syndrome virus and its inhibitory effect on caspase activity from Penaeus monodon

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• 作者：Thanunthon Bowornsakulwong^a ; Walaiporn Charoensapsri^b ; ^c ; Triwit Rattanarojpong^a ; Pongsak Khunrae^a ; ^{pongsak.khu@kmutt.ac.th}
• 关键词：WSSV134 ; Apoptosis ; White spot syndrome virus ; Caspase
• 刊名：Protein Expression and Purification
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：130
• 期：Complete
• 页码：123-128
• 全文大小：1138 K
• 卷排序：130

文摘

A functional recombinant WSSV134 was expressed and purified from E. coli expression system. The rWSSV134 protein was shown to be able to inhibit PmCasp. Site-directed mutagenesis demonstrated that residue D104 is important for PmCasp inhibition.