Generation of highly enriched V2a interneurons from mouse embryonic stem cells

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Generation of highly enriched V2a interneurons from mouse embryonic stem cells

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作者：Nisha R. Iyer^a ; James E. Huettner^b ; Jessica C. Butts^a ; Chelsea R. Brown^a ; Shelly E. Sakiyama-Elbert^a ; ^{sakiyama@wustl.edu" class="auth_mail" title="E-mail the corresponding author}
关键词：CPG ; central pattern generator ; Cyc ; cyclopamine ; DAPT ; N-{N-(3 ; 5-difluorophenacetyl-L-alanyl))-(S)-phenylglycine-t-butyl-ester ; EB ; embryoid body ; EPSC ; excitatory post synaptic current ; ESC ; embryonic stem cell ; FACS ; Fluorescence-activated cell sorting ; IN ; interneuron ; MAP2 ; microtubule associated protein 2 ; MN ; motor neuron ; PAC ; puromycin N-acetyltransferase ; pMN ; progenitor motor neuron ; Pur ; purmorphamine ; Puro ; puromycin ; RA ; retinoic acid ; SCI ; spinal cord injury ; Shh ; sonic hedgehog
刊名：Experimental Neurology
出版年：2016
出版时间：March 2016
年：2016
卷：277
期：Complete
页码：305-316
全文大小：2267 K

文摘

•: Antibiotic selection of transgenic mouse ESCs yields highly enriched V2a interneurons.
•: Purity of ESC-derived V2a IN culture persists through glutamatergic maturation.
•: Electrophysiological properties of selected V2a INs are similar to endogenous cells.
•: Selected ESC-derived V2a INs form synapses with motor neurons when co-cultured.

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