Comparison of real-time PCR and ELISA-based methods for the detection of beef and pork in processed meat products
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文摘
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Real-time PCR detected beef consistently at 0.50%, compared to 1.00% for ELISA.

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Real-time PCR detected pork consistently at 0.10%, compared to 10.0% for ELISA.

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Compared to ELISA, real-time PCR showed greater agreement among duplicate samples.

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ELISA was found to be less time consuming and easier to perform than real-time PCR.

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ELISA and real-time PCR showed 100% specificity during reference sample testing.

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