Ultrasensitive electrochemiluminescence immunosensor based on Ru(bpy)₃²⁺ and Ag nanoparticles doped SBA-15 for detection of cancer antigen 15-3

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• 作者：Xiaojian Li ; Yuyang Li ; Rui Feng ; Dan Wu ; Yong Zhang ; He Li ; Bin Du ; Qin Wei
• 关键词：Label-free ; Electrochemiluminescence immunosensor ; Ag doped SBA-15 ; Chitosan&ndash ; ionic liquid
• 刊名：Sensors and Actuators B: Chemical
• 出版年：2013
• 出版时间：November, 2013
• 年：2013
• 卷：188
• 期：Complete
• 页码：462-468
• 全文大小：1440 K

文摘

A label-free electrochemiluminescence (ECL) immunosensor based on functionalized Santa Barbara Amorphous-15 (SBA-15) has been developed for detection of cancer antigen 15-3 (CA15-3). The functionalized SBA-15 was prepared by decoration of citrate-capped Ag nanoparticles and incorporation of Ru(bpy)₃²⁺on SBA-15. The Ag nanoparticles show remarkable enhancement effect on ECL properties of Ru(bpy)₃²⁺. In order to incorporate more Ru(bpy)₃²⁺, anionic surfactant sodium dodecyl sulfate (SDS) was used to increase the negative charge of the composite matrix. Chitosan-ionic liquid (CHIT-IL) were also used to increase the conductivity and film stability. The functionalized SBA-15 was used to label the anti-CA15-3 based on the covalent interaction between Ag nanoparticles and the amines of anti-CA15-3. The ECL immunosensor that used to detect breast cancer biomarker CA15-3 exhibits high ECL intensity, good biocompatibility and long-term stability. The results show that the ECL immunosensor has high sensitivity for CA15-3 with a linear range from 0.01 U/mL to 100 U/mL and a detection limit of 0.004 U/mL (S/N = 3).