The hsa-miR-5787 represses cellular growth by targeting eukaryotic translation initiation factor 5 (eIF5) in fibroblasts

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• 作者：Hanna  ; Yoo^a ; Jung Ki  ; Yoo^a ; Jimin  ; Lee^a ; Dong Ryul  ; Lee^b ; Jung Jae  ; Ko^b ; Seoung Hun  ; Oh^c ; Young Kug  ; Choo^d ; Jin Kyeoung  ; Kim^a ;   ; ^{kyeoung66@hanmail.net}
• 关键词：Novel miRNA ; MSCs ; eIF5 ; Fibroblast ; Cellular growth inhibition
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2011
• 出版时间：2 December 2011
• 年：2011
• 卷：415
• 期：4
• 页码：567-572
• 全文大小：823 K

文摘

MicroRNAs (miRNAs) are small non-coding RNAs that regulate diverse biological processes. We cloned novel small RNA from human mesenchymal stem cells (hMSCs) and termed microRNA-5787 (hsa-miR-5787) that met the criteria for a miRNA. The level of miR-5787 was elevated in senescent fibroblasts. Based on the target prediction algorithm and results that were obtained, we find that eukaryotic translation initiation factor 5 (eIF5) is a target of miR-5787. Similar to the over-expression of miR-5787, we showed that repression of eIF5 in fibroblasts negatively affected cell growth. Therefore, we propose that the miR-5787 represses cell growth, in part, by targeting eIF5.