The early UL31 gene of equine herpesvirus 1 encodes a single-stranded DNA-binding protein that has a nuclear localization signal sequence at the C-terminus

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• 作者：Seongman Kim ; Byung Chul Ahn ; Dennis J. O¡¯Callaghan ; Seong Kee Kim
• 关键词：Equine herpesvirus 1 ; UL31 protein of EHV-1 ; Single-stranded DNA-binding protein of EHV-1 ; EHV-1 DNA replication ; Nuclear localization signal
• 刊名：Virology
• 出版年：2012
• 出版时间：25 October, 2012
• 年：2012
• 卷：432
• 期：2
• 页码：306-315
• 全文大小：1045 K

文摘

The amino acid sequence of the UL31 protein (UL31P) of equine herpesvirus 1 (EHV-1) has homology to that of the ICP8 of herpes simplex virus type 1 (HSV-1). Here we show that the UL31 gene is synergistically trans-activated by the IEP and the UL5P (EICP27). Detection of the UL31 RNA transcript and the UL31P in EHV-1-infected cells at 6 h post-infection (hpi) as well as metabolic inhibition assays indicated that UL31 is an early gene. The UL31P preferentially bound to single-stranded DNA over double-stranded DNA in gel shift assays. Subcellular localization of the green fluorescent protein (GFP)-UL31 fusion proteins revealed that the C-terminal 32 amino acid residues of the UL31P are responsible for the nuclear localization. These findings may contribute to defining the role of the UL31P single-stranded DNA-binding protein in EHV-1 DNA replication.