Bactericidal efficacy of cold plasma in processed bone. A new approach for adjuvant therapy of medication-related osteonecrosis of the jaw?

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• 作者：Shady Abu-Sirhan^a ; ¹ ; Moritz Hertel^b ; ¹ ; Saskia Preissner^a ; Henrik Carl Wirtz^a ; Sascha Rudolf Herbst^a ; Philipp Pierdzioch^a ; Jan D. Raguse^c ; Stefan Hartwig^c ; ^{stefan.hartwig@charite.de" class="auth_mail" title="E-mail the corresponding author}
• 关键词：BRONJ ; MRONJ ; Osteonecrosis ; Antiresorptive therapy ; Tissue tolerable plasma ; Cold atmospheric plasma
• 刊名：Clinical Plasma Medicine
• 出版年：2016
• 出版时间：July 2016
• 年：2016
• 卷：4
• 期：1
• 页码：9-13
• 全文大小：1185 K

文摘

Medication-related osteonecrosis of the jaw is a difficult to treat side effect of antiresorptive therapy. Despite intensive efforts in the treatment of this disease, therapy continues to be prolonged, burdensome and sometimes insufficient. Since bacterial contamination of the necrotic bone is a major factor in these problems, we have examined the antibacterial effect of physical cold atmospheric pressure plasma on contaminated bone.

Methods

A total of 72 cortico-cancellous porcine bone cylinders were processed and incubated in m>Streptococcus mitism> broth and afterwards treated with cold atmospheric plasma, chlorhexidine or sodium chloride solution (control). After grinding up the specimens, the bacteria were transferred to aqueous solution and a colony-forming unit count was performed.

Results

Physical cold atmospheric pressure plasma showed the best results in terms of reduction in colony-forming unit count in porcine bone: overall median in colony-forming unit/ml was significantly below that for chlorhexidine (m>pm>=0.004) and control (m>pm>=0.008).

Conclusion

These results suggest that cold atmospheric pressure plasma has profound effectiveness on the bacterially contaminated bone in vitro. The use on cancellous bone is possibly a promising technique and could be a useful tool in the therapeutic range for the treatment of medication-related osteonecrosis of the jaw.