Detection of pathogen Escherichia coli O157:H7 with a wireless magnetoelastic-sensing device amplified by using chitosan-modified magnetic Fe₃O₄ nanoparticles

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• 作者：Hailan Lin ; Qingzhu Lu ; Shutian Ge ; Qingyun Cai ; Craig A. Grimes
• 关键词：Escherichia coli O157 ; H7 ; Pathogen ; Biosensor ; Nanoparticle ; Wireless ; Magnetic
• 刊名：Sensors and Actuators B: Chemical
• 出版年：2010
• 出版时间：18 May 2010
• 年：2010
• 卷：147
• 期：1
• 页码：343-349
• 全文大小：1012 K

文摘

We report on a wireless magnetoelastic-sensing device for the assay of pathogen with Escherichia coli O157:H7 (E. coli) as a target using chitosan-modified magnetic Fe₃O₄ nanoparticles (CMNPs) as signal-amplifying tags. At suitable pH the CMNPs, Fe₃O₄ nanoparticles functionalized with a layer of chitosan, bind to negatively charged E. coli through electrostatic attraction. The E. coli attached CMNPs are magnetically bound to the surface of the magnetoelastic sensor, resulting in enhanced mass loading on the sensor surface that in turn decreases its resonance frequency allowing quantification of E. coli concentrations. The sensor shows a linear response to the logarithmic concentration of E. coli in the range of 10 cells mL⁻¹ to 3.7 × 10⁸ cells mL⁻¹, with a detection limit (LOD) of 10 cells mL⁻¹. Small molecules such as albumin bovine and ovalbumin show no interference on the detection.