The product of the mammalian orthologue of the Saccharomyces cerevisiae HBS1 gene is phylogenetically related to eukaryotic release factor 3 (eRF3) but does not carry eRF3-like activity

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• 作者：Wallrapp ; Christine ; Verrier ; Sidonie-Brigitte ; Zhouravleva ; Galina ; Philippe ; Herv&eacute ; et. al.
• 关键词：Translation ; Phylogeny ; HBS1 gene ; Eukaryotic release factor 3 ; Eukaryotic elongation factor 1A
• 刊名：FEBS Letters
• 出版年：1998
• 出版时间：December 4, 1998
• 年：1998
• 卷：440
• 期：3
• 页码：387-392
• 全文大小：362 K

文摘

We describe here the cloning and sequencing of human and mouse cDNAs encoding a putative GTP binding protein. Sequence comparison shows that these cDNAs (named eRFS) are likely to represent the orthologues of the yeast Saccharomyces cerevisiae HBS1 gene and that the C-terminal domains of the encoded proteins share structural features with eukaryotic elongation factor eEF-1A and release factor 3 (eRF3) families. The phylogenetic analysis suggests that eRFS proteins and Hbs1p form a cluster of orthologous sequences branching with the eRF3 family. Nevertheless, in yeast, the human eRFS protein and Hbs1p do not complement eRF3/Sup35p thermosensitive mutation and do not interact with eRF1.