Pyruvate enhances neurological recovery following cardiopulmonary arrest and resuscitation

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• 作者：Arti B. Sharma ; Matthew A. Barlow ; Shao-Hua Yang ; James W. Simpkins ; Robert T. Mallet
• 关键词：Brain ischemia ; Cardiac arrest ; Cardiopulmonary resuscitation (CPR) ; Cerebral blood flow ; Inflammatory response ; Metabolism ; Neurological dysfunction ; Post-resuscitation period
• 刊名：Resuscitation
• 出版年：2008
• 出版时间：January 2008
• 年：2008
• 卷：76
• 期：1
• 页码：108-119
• 全文大小：1227 K

文摘

Cerebral oxidative stress and metabolic dysfunction impede neurological recovery from cardiac arrest-resuscitation. Pyruvate, a potent antioxidant and energy-yielding fuel, has been shown to protect against oxidant- and ischemia-induced neuronal damage. This study tested whether acute pyruvate treatment during cardiopulmonary resuscitation can prevent neurological dysfunction and cerebral injury following cardiac arrest.

Methods

Anesthetized, open-chest mongrel dogs underwent 5 min cardiac arrest, 5 min open-chest cardiac compression (OCCC), defibrillation and 3-day recovery. Pyruvate (n = 9) or NaCl volume control (n = 8) were given (0.125 mmol kg⁻¹ min⁻¹ i.v.) throughout OCCC and the first 55 min recovery. Sham dogs (n = 6) underwent surgery and recovery without cardiac arrest-resuscitation.

Results

Neurological deficit score (NDS), evaluated at 2-day recovery, was sharply increased in NaCl-treated dogs (10.3 ± 3.5) versus shams (1.2 ± 0.4), but pyruvate treatment mitigated neurological deficit (NDS = 3.3 ± 1.2; P < 0.05 versus NaCl). Brain samples were taken for histological examination and evaluation of inflammation and cell death at 3-day recovery. Loss of pyramidal neurons in the hippocampal CA1 subregion was greater in the NaCl controls than in pyruvate-treated dogs (11.7 ± 2.3 % versus 4.3 ± 1.2 % ; P < 0.05). Cardiac arrest increased caspase-3 activity, matrix metalloproteinase activity, and DNA fragmentation in the CA1 subregion; pyruvate prevented caspase-3 activation and DNA fragmentation, and suppressed matrix metalloproteinase activity.

Conclusion

Intravenous pyruvate therapy during cardiopulmonary resuscitation prevents initial oxidative stress and neuronal injury and enhances neurological recovery from cardiac arrest.