Cell adhesion on supported lipid bilayers functionalized with RGD peptides monitored by using a quartz crystal microbalance with dissipation

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• 作者：Xiaohua Zhu^a ; ^b ; Zhao Wang^a ; ^b ; Ansha Zhao^a ; ^b ; ^{anshazhao@263.net" class="auth_mail} ; Nan Huang^a ; ^b ; Huadong Chen^c ; Shuo Zhou^a ; ^b ; Xiao Xie^d
• 关键词：SLBs ; QCM-D ; Cell adhesion ; RGD ; Dynamic monitoring
• 刊名：Colloids and Surfaces B: Biointerfaces
• 出版年：1 April, 2014
• 年：2014
• 卷：116
• 期：Complete
• 页码：459-464
• 全文大小：1254 K

文摘

Supported lipid bilayer (SLB) is one of the most widely used structures to mimic cell membranes. To study the cell-cell, cell-matrix and cell-material interactions, supported lipid bilayers (SLBs) functionalized with RGD peptides (SLBs-RGD) were prepared by vesicle fusion on a SiO₂ quartz crystal, and subsequently bone mesenchymal stem cells (BMSCs) adhesion was analyzed. A quartz crystal microbalance with dissipation (QCM-D) was utilized to detect the dynamic adsorption behavior of lipid vesicles and BMSCs in real time. Observations obtained by QCM-D signals are confirmed by conducting fluorescence microscopy.

QCM-D measurements showed the SLB formation starts at the critical concentration of the vesicles. More BMSCs adhered on SLBs-RGD than on SLBs. With the presence of SLBs, the adhesion cells on SLBs surfaces had a rounded morphology, and cells on SLBs-RGD will take long time to rearrange their cytoskeleton, which led to incomplete spreading compared with SiO₂. Differences in adhesion density and adhesion properties of the cells on the different substrates could be traced at the dissipation versus frequency (螖D/螖f) plots. These results indicate that RGD in/on SLBs could provide anchorage sites for more cells adhesion. QCM-D is demonstrated to be a useful tool for evaluating the interactions between various biological and non-biological systems in situ and in real-time.