Cloning, expression, and characterization of a thermostable and pH-stable laccase from Klebsiella pneumoniae and its application to dye decolorization

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• 作者：Yihan Liu^a ; ^c ; ^d ; ¹ ; Lin Huang^a ; ^b ; ^d ; ¹ ; Wei Guo^a ; ^d ; Leibo Jia^b ; ^d ; Yu Fu^d ; Shuang Gui^a ; ^d ; Fuping Lu^a ; ^c ; ^d ; ^{lfp@tust.edu.cn}
• 关键词：Laccase ; Klebsiella pneumoniae ; Enzyme property ; Circular dichroism spectroscopy ; Dye decolorization
• 刊名：Process Biochemistry
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：53
• 期：Complete
• 页码：125-134
• 全文大小：2469 K
• 卷排序：53

文摘

A novel laccase from Klebsiella pneumoniae was cloned and the rLac was expressed in Escherichia coli. The rLac was thermostable and pH stable and resistant toward metal ions. The structure–function relationship of rLac under different conditions was analyzed by far-UV CD spectroscopy. The rLac could decolorize tested dyes with high decolorization efficiencies.