Sensing of biomolecules and label-free discrimination of DNA containing a triple T-C/T-G mismatch pair with a fluorescence light-up probe, triazolylpyrene (^TNDMBPy)

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• 作者：Subhendu Sekhar Bag ; ^{ssbag75@iitg.ernet.in} ; Rajen Kundu ; Subhashis Jana
• 关键词：Triazolylpyrene ; Fluorescence light-up probe ; Sensing biomolecules ; ct-DNA ; BSA ; Label free mismatched DNA detection
• 刊名：Tetrahedron Letters
• 出版年：2013
• 出版时间：22 May, 2013
• 年：2013
• 卷：54
• 期：21
• 页码：2627-2632
• 全文大小：682 K

文摘

Binding to the minor groove of calf-thymus DNA (ct-DNA) and strong binding in hydrophobic pocket of bovine serum albumin (BSA), switched-on the fluorescence of smart fluorescent probe, triazolylpyrene (^TNDMBPy). Also, a novel label-free strategy was adopted to detect DNA base mismatch via the generation of distinct fluorescence signal at visible region utilizing the same probe