文摘
Six piglets aged 20 days were inoculated oranasally with 5 ml of a suspension (106 TCID50/ml) of a Greek myocardial strain of encephalomyocarditis virus (EMCV). The animals either died (n=2) or were killed for examination on days 1,2 or 3 post-inoculation (pi). EMCV was isolated from virtually all organs examined (heart, tonsils, palatine glands, pancreas, spleen, small intestine and mesenteric lymph nodes). Histopathologically, interstitial myocarditis, necrosis of cardiac muscle cells and Purkinje fibres, and necrotizing tonsillitis were detected in all inoculated piglets. Focal interstitial pancreatitis, necrosis of pancreatic acinar cells and Langerhans islet cells, and necrosis of germinal centre lymphocytes of the lymph nodes and Peyer's patches were detected in two piglets that died or were killed on day 3 pi. Immunohistochemically, viral antigen was detected in epithelial cells of all organs examined, including the tonsils, palatine glands, pancreatic interlobular ducts and small intestine. This suggests that EMCV is epitheliotropic, in addition to its known myocardial tropism. The frequent presence of intracytoplasmic EMCV in macrophages of the tonsils and spleen supports the hypothesis that macrophages play a role in viral replication and dissemination in the body.
