Functional heterologous expression and characterization of mannuronan C5-epimerase from the brown alga Saccharina japonica

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Functional heterologous expression and characterization of mannuronan C5-epimerase from the brown alga Saccharina japonica

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作者：Akira Inoue^a ; ^{inouea21@fish.hokudai.ac.jp" class="auth_mail" title="E-mail the corresponding author} ; Aya Satoh^a ; Mio Morishita^a ; Yuko Tokunaga^a ; Takuya Miyakawa^b ; Masaru Tanokura^b ; Takao Ojima^a
关键词：Alginate ; Mannuronan C5-epimerase ; Brown algae ; Alginate biosynthesis ; Cell wall
刊名：Algal Research
出版年：2016
出版时间：June 2016
年：2016
卷：16
期：Complete
页码：282-291
全文大小：2050 K

文摘

•: Brown algal MC5E was successfully expressed using insect cells.
•: Purified MC5E alternately epimerized β-d-mannuroic acid to α-l-guluronic acid.
•: This is the first report on functional expression of eukaryotic MC5E.

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